Related Experiment Video
Updated: Jun 19, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
Published on: January 6, 2026
Increase in depth of field taking into account deconvolution by optimization of pupil mask
Frédéric Diaz1, François Goudail, Brigitte Loiseaux
1Thales Research & Technology, 1 Avenue Augustin Fresnel, 91767 Palaiseau CEDEX, France. frederic.diaz@thalesgroup.com
Abstract:
We consider optimization of hybrid imaging systems including a phase mask for enhancing the depth of field and a digital deconvolution step. We propose an image quality criterion that takes into account the variability of the system's point-spread function along the expected defocus range and the noise enhancement induced by deconvolution. Considering the classical cubic phase mask as an example, we show that the optimization of this criterion may lead to filter parameters that are significantly different from those usually proposed to ensure the strict invariance of the PSF.
Related Concept Videos
Deconvolution
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Focusing of Light in the Eye
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy
Depth Perception and Spatial Vision
Convolution Properties II
The width property indicates that if the durations of input signals are T1 and T2, then the width of the output response equals the sum of both durations, irrespective of the shapes of the two functions. For instance, convolving two rectangular pulses with durations of 2 seconds and 1 second results in a function with a width of 3 seconds.
The area property asserts that the area under the...
