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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: Jun 19, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

[Immunohistochemical algorithms in prostate diagnostics: what's new?].

G Kristiansen¹

¹Department Pathologie, Institut für klinische Pathologie, Universitätsspital Zürich (USZ), Schmelzbergstr 12, 8091 Zürich, Schweiz. glen.kristiansen@usz.ch

|October 2, 2009

Summary

This summary is machine-generated.

Immunohistochemistry aids prostate cancer diagnosis by identifying basal cell loss. New markers like GOLPH2 and FASN could improve accuracy alongside established tools such as alpha-methylacyl-CoA racemase (AMACR).

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Last Updated: Jun 19, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

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Published on: April 8, 2016

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Detection and Isolation of Cancer in Prostate Biopsies Using Stimulated Raman Histology and Artificial Intelligence

Detection and Isolation of Cancer in Prostate Biopsies Using Stimulated Raman Histology and Artificial Intelligence

Published on: June 10, 2025

Area of Science:

Uropathology
Molecular Pathology
Cancer Biomarkers

Context:

Immunohistochemistry is crucial for prostate biopsy diagnosis.
Basal cell markers (e.g., p63) help distinguish benign from malignant prostate tissues, as their absence indicates malignancy.
Current diagnostic methods benefit from positive markers to enhance accuracy.

Purpose:

To critically review existing prostate cancer immunohistochemistry biomarkers.
To explore the potential of novel markers for improved diagnostic accuracy in prostate cancer.
To suggest Golgi phosphoprotein 2 (GOLPH2) and Fatty acid synthase (FASN) as additional diagnostic markers.

Summary:

Basal cell markers like p63 are vital in prostate cancer diagnostics, with their absence signifying malignancy.
Alpha-methylacyl-CoA racemase (AMACR) is a widely used positive marker, often combined with p63.
Golgi phosphoprotein 2 (GOLPH2) and Fatty acid synthase (FASN) are proposed as supplementary positive markers to increase diagnostic precision.

Impact:

Enhances the diagnostic toolkit for genito-urinary pathologists.
Aims to improve the accuracy of prostate cancer diagnosis through novel biomarker identification.
Facilitates more precise differentiation between benign and malignant prostate lesions.