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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Poly (N-isopropylacrylamide)-coated multifunctional nanoparticles for cell tracking.

Pu Wang¹, Jia He, Pei-Nan Wang

¹Surface Physics Laboratory (National Key Laboratory) and Department of Physics, Fudan University, Shanghai, China.

Photomedicine and Laser Surgery

|October 3, 2009

Summary

Multifunctional nanoparticles offer a novel method for tracking cells. These nanoparticles enable dual fluorescence and magnetic resonance imaging for reliable in vitro and in vivo cell visualization.

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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

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Magnetic and Thermal-sensitive Poly(N-isopropylacrylamide)-based Microgels for Magnetically Triggered Controlled Release

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Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability

Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability

Published on: February 27, 2016

Area of Science:

Biotechnology
Nanomedicine
Cell Biology

Background:

Effective cell tracking is crucial for understanding cell therapeutics.
Existing cell tracking methods have limitations.
A novel approach using multifunctional nanoparticles for dual-mode tracking is explored.

Purpose of the Study:

To investigate a new cell tracking modality using multifunctional nanoparticles.
To evaluate the efficacy of these nanoparticles for in vitro and in vivo cell tracking.

Main Methods:

Poly(N-isopropylacrylamide) (PNIPAM)-coated nanoparticles with quantum dots (QDs) and Fe(3)O(4) magnetic particles were synthesized.
Chinese hamster ovary (CHO) cells were labeled with these nanoparticles.
In vitro tracking utilized confocal fluorescence microscopy; in vivo tracking employed magnetic resonance imaging (MRI).

Main Results:

Nanoparticles demonstrated stable, non-toxic binding to CHO cell membranes.
Fluorescence was detectable for over 48 hours post-labeling.
Labeled cells were successfully visualized and manipulated magnetically in vivo using MRI.

Conclusions:

PNIPAM-coated multifunctional nanoparticles are effective for cell labeling.
This dual-mode (fluorescence and MRI) tracking system is reliable for both in vitro and in vivo applications.
The method offers simplicity and dependability for advanced cell tracking.