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Updated: Jun 19, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban
Ok Tae Kim1, Jei Wan Lee, Kyong Hwan Bang
1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-871, Republic of Korea. kimot99@hanmail.net
Abstract:
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.
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