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Related Experiment Video

Updated: Jun 19, 2026

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

Published on: May 26, 2021

Essential role for Pbx1 in corneal morphogenesis.

Mark J Murphy¹, Bozena K Polok, Daniel F Schorderet

¹Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.

Investigative Ophthalmology & Visual Science

|October 3, 2009

Summary

The Pbx1 proto-oncogene is essential for corneal development. Its deletion in mice causes severe corneal dystrophy, highlighting its role in maintaining corneal structure and function.

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Area of Science:

Developmental Biology
Ophthalmology
Genetics

Background:

Pbx TALE homeodomain proteins interact with Hox proteins, crucial for cell fate.
Pbx1 is a key regulator in developmental processes.

Purpose of the Study:

To investigate the function of Pbx1 in the mouse corneal epithelium.
To understand the role of Pbx1 in corneal morphogenesis.

Main Methods:

Used Pbx1(f/f) mice crossed with K14-Cre mice for tissue-specific deletion.
Analyzed eyes via histologic and molecular methods.

Main Results:

Pbx1 deletion caused corneal dystrophy and clouding from birth, worsening with age.
Observed corneal epithelial thickening, stromal infiltration by atypical basal cells, and collagen disorganization.
Found high epithelial cell turnover, perturbed developmental regulators, and aberrant differentiation.

Conclusions:

Pbx1 is essential for normal corneal development and morphogenesis.
Pbx1 plays a critical role in maintaining corneal structure and barrier function.