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Fate of ingested immunoglobulin G in the mosquito Aedes aegypti (Diptera: Culicidae)
1Department of Parasitology, Kaohsiung Medical College, Taiwan, Republic of China.
Abstract:
The amount of bloodmeals ingested by Aedes aegypti was estimated to be 3.00 +/- 1.05 mg (2.84 +/- 0.99 microliters) in average. The feeding time of a mosquito was not necessarily associated with the quantity of the bloodmeal. Nevertheless, the number of egg laid after bloodfeeding was apparently dependent on the volume of ingested bloodmeals. The change of immunoglobulin G (IgG) in the ingested bloodmeals of Aedes aegypti was also evaluated by ELISA. To detect the survival of ingested IgG, 5-day-old adult female mosquitoes were allowed to feed on a human arm. After completion of feeding, 3 engorged mosquitoes were selected on the basis of 6-hour intervals until the 54th hour post ingestion. Selected specimens were deprived of their legs and wings, homgenized and centrifuged. The supernatants were collected for ELISA procedure. The control groups included the blank (PBS) and unfed mosquitoes. All OD405 values representing the amount of IgG in the ingested bloodmeal were evaluated as 1.702 +/- 0.017, 0.469 +/- 0.046, 0.414 +/- 0.084, 0.231 +/- 0.012, 0.180 +/- 0.025, 0.172 +/- 0.006, 0.163 +/- 0.012, 0.173 +/- 0.007, 0.168 +/- 0.012, and 0.169 +/- 0.014, respectively for each interval. A blood sample taken from the same person fed to mosquitoes was evaluated to be 1.716 +/- 0.012. The OD405 values of the blank and the unfed group were 0.156 +/- 0.019 and 0.159 +/- 0.009, respectively. The result showed that IgG ingested by Aedes aegypti may be digested vigorously within 6 hours.(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
Aedes aegypti mosquitoes ingest an average of 3 mg of blood, influencing egg-laying. Ingested immunoglobulin G (IgG) is rapidly digested within 6 hours post-feeding.
Area of Science:
- Medical Entomology
- Immunology
Background:
- Aedes aegypti mosquitoes are significant vectors of diseases.
- The quantity of bloodmeal ingested by mosquitoes can influence their reproductive capacity.
- The survival and digestion of immunoglobulins in the mosquito's gut are not well understood.
Purpose of the Study:
- To quantify the bloodmeal volume ingested by Aedes aegypti.
- To investigate the relationship between bloodmeal volume and egg production.
- To determine the survival and digestion rate of immunoglobulin G (IgG) in Aedes aegypti after feeding.
Main Methods:
- Quantified bloodmeal volume in Aedes aegypti.
- Monitored egg laying in relation to bloodmeal size.
- Used ELISA to measure IgG levels in engorged mosquitoes at 6-hour intervals post-feeding for up to 54 hours.
- Included blank and unfed mosquito controls.
Main Results:
- Average bloodmeal size was 3.00 +/- 1.05 mg.
- Egg production correlated with ingested bloodmeal volume.
- ELISA results indicated a significant decrease in IgG levels within 6 hours post-ingestion, suggesting rapid digestion.
Conclusions:
- Bloodmeal volume is a key factor for Aedes aegypti egg production.
- Ingested immunoglobulin G (IgG) is subject to rapid digestion in Aedes aegypti, likely within 6 hours.