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Attovial-based antibody nanoarrays

Peter Ellmark1, Sara Ghatnekar-Nilsson, André Meister

  • 1Department of Immunotechnology, Lund University, Lund, Sweden.

Proteomics
|October 3, 2009
PubMed

Antibody array-based technology is a powerful emerging tool in proteomics, but to enable global proteome analysis, antibody array layouts with even higher density has to be developed. To this end, we have further developed the first generation of a nanoarray platform, based on attoliter-sized vials, attovials, which we have characterized and used for the detection of complement factor C1q in human serum samples. Finally, we demonstrated proof-of-concept for individual functionalization of the attovials with a recombinant antibody.

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