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Updated: Jun 19, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
[The self intensification of the pure spectrum in the mixed spectrum]
Zhi-Ping Wang1, Yan-Lin Tang, Fu-Rong Zhang
1College of Science, Guizhou University, Guiyang 550025, China. hnwzp1982@126.com
Abstract:
In the present paper, the influence of the pure spectrum contained in the mixed spectrum was centralized at the origin, and in this way, the influence of the pure spectrum in the result curve reached the maximum value. This process is called the self intensification of the pure spectrum. Farther, considering that the center of the Gaussian function contained in the impure spectrum is much different from that of the pure spectrum, it can be accepted that the influence of the impure spectrum at the origin is approximately the minimum. So the origin was chosen as the analysis point when the authors perform the quantitative analysis according to the spectrum. Therefore we can reduce the subjectivity in choosing the analysis point. In addition, the wavelet coefficients of the result curve at the origin were used for the quantitative analysis. According to the simulated experiment, it was found that the final result is independent of the Gaussian white noise contained in the actual spectrum. Finally, the analytic result of the new method was compared with that of the method reported in the literature, which proved that the new method is much better in terms of the analytic error.
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