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Updated: Jun 19, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
[Simple sequence repeat variation and small-scale spatial autocorrelation analysis on smooth-shell populations of
Jun-tao Guo1, Yi-biao Zhou, Zhi-jie Zhang
1Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Objective:
To analysis the spatial autocorrelation on the small-scale distribution of the genetic variation in the population of Oncomelania hupensis in Puge county, Sichuan province, using simple sequence repeat (SSR) marker.
Methods:
5 pairs of SSR primer were used to amplify the genomic DNA of Oncomelania hupensis, and the alleles with frequency ranging from 15% to 85% were used to calculate Moran's I spatial autocorrelation coefficients in 14 distance band based on equal numbers of paired samples.
Results:
A total of 274 alleles were scored by 5 pairs of SSR primer, the average polymorphic information content of the 274 alleles were 0.965 which indicated a high level of genetic diversity. 39 alleles showed different patterns of positive spatial autocorrelation of genetic variation, which was non-random spatial structure. When the distance band increased, the spatial auto-correlativity decreased based on the average Moran's I value at 14 distance band. The alleles which showed a negative spatial autocorrelation were not found in any distance band.
Conclusion:
The spatial distribution of the genetic variation of SSR showed positive spatial autocorrelation in the population of Oncomelania hupensis, and the spatial auto-correlativity decreased with the increase of distance band.
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