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Updated: Jun 19, 2026

08:49
Freezing and Thawing Human Embryonic Stem Cells
Published on: December 24, 2009
[Does 2009 mark a revival of embryonic stem cells?]
1Departamento Interfacultativo de Bioquímica y Biología Molecular, Universidad de Navarra, 31080 Pamplona. Spain. natalialm@unav.es
Summary
Federal funding for embryonic stem cell research in the US began in 2009, focusing on cell banks rather than regenerative therapies. The study explores alternatives to embryonic cells for advancing induced pluripotent stem cell research.
Area of Science:
- Biomedical Research
- Stem Cell Biology
- Biotechnology
Context:
- US federal funding for embryonic cell research and embryo destruction became legal in March 2009.
- This funding is directed towards creating human cell banks, primarily for biomedical research, not regenerative therapies.
- The initiative involves scientists linked to biotechnology enterprises and in vitro fertilization centers.
Purpose:
- To analyze the legal landscape and scientific implications of using federal funds for embryonic cell research.
- To critically evaluate the reliance on embryonic stem cells for creating cell lines.
- To explore and advocate for alternative methods in evaluating induced pluripotent stem (iPS) cells.
Summary:
- The study examines the 2009 US policy shift allowing federal funding for research involving embryonic cells and embryo destruction.
- It highlights the focus on establishing human cell banks for research purposes, often driven by commercial interests.
- The abstract discusses the widespread use of embryonic cell lines and the potential of iPS cells, emphasizing the need for ethical and scientifically sound alternatives that avoid embryo destruction.
Impact:
- The research encourages the adoption of rational alternatives to embryonic cell use in evaluating iPS cells.
- It opens new avenues for cell therapy research by validating reprogrammed somatic cells.
- The findings contribute to the ethical and scientific discourse surrounding stem cell research and its funding.
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Embryonic Stem Cells
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ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

