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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels.

Xin Fu1, Kelong Huang, Suqin Liu

  • 1Department of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. fuxin1129@hotmail.com

Journal of Microbiological Methods
|October 6, 2009
PubMed
Summary

A new fluorescence method uses cadmium selenide/zinc sulfide (CdSe/ZnS) nanoparticles to rapidly detect bacterial counts. This sensitive technique offers a low detection limit for bacterial quantification.

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Last Updated: Jun 19, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

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Published on: August 7, 2016

Solubilization and Bio-conjugation of Quantum Dots and Bacterial Toxicity Assays by Growth Curve and Plate Count
13:06

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Published on: July 11, 2012

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17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Area of Science:

  • Nanotechnology
  • Microbiology
  • Analytical Chemistry

Background:

  • Accurate bacterial quantification is crucial for various fields, including clinical diagnostics and environmental monitoring.
  • Traditional methods for bacterial enumeration can be time-consuming and labor-intensive.
  • Development of rapid and sensitive detection techniques is highly desirable.

Purpose of the Study:

  • To develop a rapid and sensitive fluorescence measurement method for bacterial detection.
  • To utilize cadmium selenide/zinc sulfide (CdSe/ZnS) as a fluorescence marker for bacterial quantification.
  • To establish the correlation between fluorescence intensity and bacterial count.

Main Methods:

  • Synthesis of high-quality CdSe/ZnS nanocrystals.
  • Conjugation of CdSe/ZnS nanocrystals with bacterial cells.
  • Measurement of fluorescence intensity and correlation with bacterial concentration.

Main Results:

  • Successful synthesis and conjugation of CdSe/ZnS nanocrystals with bacteria.
  • Demonstrated a linear relationship between fluorescence intensity and bacterial count over a range of 10(2)-10(8) CFU/mL.
  • Achieved a low detection limit of 10(2) CFU/mL for bacterial enumeration.

Conclusions:

  • The developed fluorescence method provides a rapid and sensitive approach for bacterial detection.
  • CdSe/ZnS nanocrystals serve as effective fluorescence markers for bacterial quantification.
  • This method holds potential for applications requiring quick and accurate bacterial count determination.