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Updated: Jun 19, 2026

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
Published on: July 20, 2022
Coenzyme A enhances activity of the mitochondrial adenine nucleotide translocator
Erika Cione1, Attilio Pingitore, Francesco Genchi
1Department of Pharmaco-Biology, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy.
Abstract:
The adenine nucleotide translocator (ANT) accomplishes the exchange of ATP from the mitochondrial matrix with cytoplasmic ADP. While investigating the biochemical mechanism of retinoic acid (RA) on the ANT via retinoylation, we have found and subsequently demonstrated a positive influence of Coenzyme A (CoA) on the transport of ATP across the membranes of rat liver mitochondria. CoA enhances ANT activity in a dose-dependent manner modifying the V(max) (673.3+/-20.7 nmol ATP/mgprotein/min versus 155.0+/-1.9 nmol ATP/mgprotein/min), the IC(50) for the specific inhibitor carboxyatractyloside (CATR) (0.142+/-0.012 microM versus 0.198+/-0.011 microM) but not the K(m) (22.50+/-0.52 microM versus 22.19+/-0.98 microM). Data suggest a likely enzymatic involvement in the interaction between ANT and CoA. The effect of CoA is observed in mitochondria from several different tissues.
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