Jove
Visualize
Contact Us

Related Experiment Video

Updated: Jun 19, 2026

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

Whole cell biosensing via recA::mCherry and LED-based flow-through fluorometry.

R L Martineau1, V Stout, B C Towe

  • 1Harrington Department of Bioengineering, Arizona State University, Tempe, AZ 85287-9709, USA. Rhett.Martineau@gmail.com

Biosensors & Bioelectronics
|October 6, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical tracking of a stress-responsive gene amplifier applied to cell-based biosensing and the study of synthetic architectures.

Biosensors & bioelectronics·2010
Same author

Molecular genetics and genomic analysis of scytonemin biosynthesis in Nostoc punctiforme ATCC 29133.

Journal of bacteriology·2007
Same author

Evaluation of microfluidic blood gas sensors that combine microdialysis and optical monitoring.

Medical & biological engineering & computing·2004
Same author

Microbial growth in a steady-state model of ethylene glycol-contaminated soil.

Current microbiology·1998
Same author

Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12.

Journal of bacteriology·1997
Same author

Investigation of a Lorentz force biomagnetometer.

IEEE transactions on bio-medical engineering·1997
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

A new optical cell enables whole-cell biosensors using red fluorescent protein (RFP) for sensitive DNA damage detection. This compact system minimizes distortions in dense samples, improving accuracy for environmental monitoring.

Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Optical Engineering

Background:

  • Whole-cell biosensors require sensitive detection methods.
  • Existing optical systems can suffer from distortions in dense samples.

Purpose of the Study:

  • Develop a miniature, cost-effective optical cell for biosensor integration.
  • Compare red fluorescent protein (RFP) and green fluorescent protein (GFP) as reporters.
  • Establish a method for simultaneous fluorescence and biomass measurement.

Main Methods:

  • Designed a compact optical system with LEDs, photodiodes, and filters.
  • Utilized Escherichia coli with recA promoter-fusions to GFP and RFP.
  • Exposed cultures to the DNA-damaging agent mitomycin C (MMC).

More Related Videos

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

Related Experiment Videos

Last Updated: Jun 19, 2026

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

  • Developed a multi-channel calibration method for optical data.

    • Main Results:

    • RFP (mCherry) showed fewer measurement distortions than GFP (GFPmut3.1) in dense cultures.
    • Lower limit of detection for MMC: 0.25nM (recA::mCherry) vs. 2.0nM (recA::gfpmut3.1).
    • The integrated calibration method effectively eliminated optical distortions, negating sample dilution.

    Conclusions:

    • The developed optical cell is suitable for disposable biosensor platforms.
    • RFP is a superior reporter protein for this compact biosensor system.
    • The novel calibration method enhances accuracy and simplifies sample handling.