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Updated: Jun 19, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Rapid analysis and sensitive detection of DL-tryptophan by using shorter capillary column coupled with deep-UV
Gamze Kavran Belin1, Valeria Gärtner, Stefan Seeger
1University of Zurich, Institute of Physical Chemistry, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. g.belin@pci.unizh.ch
Abstract:
A laboratory made capillary electrophoresis (CE) system using shorter capillary column was designed and constructed in our research group. This promising CE system was then connected with confocal fluorescence microscopy which was also developed in our research group. In order to show the applicability of this new system on chiral separation of DL-tryptophan (DL-Trp), studies were undertaken using cyclodextrins (CDs) and their derivatives; sulfated (S-CDs) and highly sulfated cyclodextrins (HS-CDs) as chiral selectors. Different selector concentrations were tested at various pH levels (pH 2.5, 4.0, 6.0, 9.0) to optimize the separation conditions. The best results were achieved at lower pH values (e.g. pH 2.5 and pH 4.0). HS-beta-CD and HS-gamma-CD were found to be the most effective chiral selectors with a shorter retention time at low selector concentrations.
