Reproductive and diurnal rhythms regulate vocal motor plasticity in a teleost fish

Seasonal and circadian rhythms control fundamental physiological processes including neural excitability and synaptic plasticity that can lead to the periodic modulation of motor behaviors like social vocalizations. Parental male midshipman fish produce three call types during the breeding season: long duration (min to >1 h) advertisement 'hums', frequency and amplitude modulated agonistic 'growls' (s), and very brief (ms) agonistic 'grunts' produced either singly or repetitively as ;grunt trains' for up to several minutes. Fictive grunts that establish the temporal properties of natural grunts are readily evoked and recorded in vivo from vocal occipital nerve roots at any time of day or year by electrical microstimulation in either the midbrain periaqueductal gray or a hindbrain vocal pre-pacemaker nucleus. Now, as shown here, the longer duration fictive growls and hums can also be elicited, but are restricted to the nocturnal reproductive season. A significant drop in call threshold accompanies the fictive growls and hums that are distinguished by their much longer duration and lower and more regular firing frequency. Lastly, the long duration fictive calls are dependent upon increased stimulation time and intensity and hence may result from activity-dependent changes in the vocal motor circuit that are themselves modulated by seasonal and circadian rhythms.