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Updated: Jun 19, 2026

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade
Alison Buchan1, Mary Hadden, Marcelino T Suzuki
1Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA. abuchan@utk.edu
Abstract:
Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.
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