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Cloning and mapping of the potato virus Y genome and its in vitro expression
1Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
Virus Genes
|September 1, 1990
Abstract:
Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.