Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Step-like concave retroreflector for single pulse Compton backscattering.

Optics express·2025

Same author

Characteristics of Mandibular Canal Branches Related to Nociceptive Marker.

Journal of dental research·2021

Same author

Phase control of a z-current-driven plasma column.

Physical review. E·2020

Same author

Non-uniformity smoothing of direct-driven fuel target implosion by phase control in heavy ion inertial fusion.

Scientific reports·2019

Same author

Peculiar behavior of Si cluster ions in a high-energy-density solid Al plasma.

Physical review. E·2019

Same author

Light-tunable Fano resonance in metal-dielectric multilayer structures.

Scientific reports·2016

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Speckle-free image in a laser-diode microscope by using the optical feedback effect.

B Dingel, S Kawata

|October 6, 2009

Summary

This study introduces a novel laser-diode microscope that eliminates speckle noise for clearer images. By using a laser diode

Area of Science:

Optical microscopy
Laser physics
Image processing

Background:

Lasers offer advantages in microscopy for observing dynamic phenomena due to controllable properties.
Speckle noise significantly degrades image quality in laser-based microscopy.
Existing methods for speckle reduction often involve complex or bulky components.

Purpose of the Study:

To develop a laser-diode microscope capable of generating speckle-free images.
To overcome the limitations of speckle noise in high-resolution dynamic imaging.
To provide a simple and effective solution for speckle reduction in microscopy.

Main Methods:

Utilizing the feedback effect in a laser diode to induce multimode operation and spectral instability.
Employing a multimode fiber to scramble the laser output, creating a time-varying speckle pattern.

More Related Videos

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Related Experiment Videos

Last Updated: Jun 19, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Averaging uncorrelated speckle patterns with a video detector to achieve speckle noise reduction.

Main Results:

Demonstration of a fiber-illuminated laser-diode microscope that effectively suppresses speckle noise.
Achieved speckle reduction without mechanical parts or additional complex optical elements.
Experimental validation showing excellent speckle reduction capabilities.

Conclusions:

The proposed fiber-illuminated laser-diode microscope provides a robust method for generating speckle-free images.
This technique offers a significant improvement in image quality for laser microscopy applications.
The simplicity and efficiency of the method make it suitable for various microscopic imaging scenarios.