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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Abiotic stress-associated miRNAs: detection and functional analysis.

Dong-Hoon Jeong1, Marcelo A German, Linda A Rymarquis

  • 1Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 6, 2009
PubMed
Summary

MicroRNAs (miRNAs) regulate plant development and respond to environmental stress. This chapter details methods for identifying stress-associated miRNAs and their gene targets using deep sequencing.

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

  • Plant molecular biology
  • Genomics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small regulatory RNA molecules crucial for posttranscriptional gene regulation in plants.
  • They are essential for normal plant development and have been implicated in responses to environmental challenges.
  • Abiotic stresses significantly impact plant growth and crop yield, necessitating research into regulatory mechanisms.

Purpose of the Study:

  • To describe methodologies for identifying and characterizing microRNAs involved in plant responses to abiotic stress.
  • To outline techniques for pinpointing the specific target genes regulated by these stress-associated miRNAs.
  • To provide a comprehensive guide for researchers in the field of plant small RNA biology.

Main Methods:

  • Small RNA cloning techniques to capture and isolate small RNA molecules.
  • High-throughput deep sequencing (NGS) to generate comprehensive expression profiles of known and novel miRNAs.
  • Bioinformatic analyses for miRNA identification, target prediction, and validation.

Main Results:

  • Identification of known and novel microRNAs responsive to various abiotic stress conditions.
  • Characterization of the expression patterns of these miRNAs under stress.
  • Determination of potential target genes regulated by the identified abiotic stress-associated miRNAs.

Conclusions:

  • Deep sequencing and small RNA cloning are powerful tools for discovering stress-responsive miRNAs in plants.
  • Understanding miRNA-mediated regulation is key to developing crops with enhanced abiotic stress tolerance.
  • This chapter provides a foundational methodology for future research in plant stress-related small RNAs.