[Determination of xanthohumol in beer by solid-phase extraction-high performance liquid chromatography]

Area of Science:

• Analytical Chemistry
• Chromatography
• Food Science

Context:

• Xanthohumol is a prenylated chalcone found in hops, known for its potential health benefits.
• Accurate quantification of xanthohumol in beer is crucial for quality control and understanding its contribution to beer's properties.
• Existing methods for xanthohumol determination may be complex or time-consuming.

Purpose:

• To develop and validate a convenient and accurate method for determining xanthohumol content in beer.
• To establish a reliable analytical procedure using solid-phase extraction coupled with high-performance liquid chromatography (SPE-HPLC).

Summary:

• A novel SPE-HPLC method was developed for xanthohumol determination in beer.
• The method utilizes a Waters Sep-Pak C18 column for sample preparation and an Agilent Zorbax Eclipse XDB-C18 column for separation.
• Optimized chromatographic conditions (linear gradient, mobile phase composition, flow rate, temperature, and detection wavelength) ensure efficient separation and detection.

Impact:

• The developed method demonstrates excellent linearity (r² = 1) over a wide concentration range (0.5–500 µg/L).
• High average recoveries (91.21%–95.58%) and low relative standard deviations (<2%) confirm the method's accuracy and precision.
• The method's low limit of detection (0.24 µg/L) and limit of quantification (0.80 µg/L) make it suitable for routine analysis of xanthohumol in beer.