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Updated: Jun 19, 2026
An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
Published on: February 22, 2010
Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer
Sini Junttila1, Kean-Jin Lim, Stephen Rudd
1Centre for Biotechnology, Tykistökatu 6 A, 20520 Turku, Finland. sini.junttila@btk.fi
Background:
The reindeer lichen is the product of a mutualistic relationship between a fungus and an algae. Lichen demonstrate a remarkable capacity to tolerate dehydration. This tolerance is driven by a variety of biochemical processes and the accumulation of specific secondary metabolites that may be of relevance to the pharmaceutical, biotechnology and agriculture industries. These protective metabolites hinder in vitro enzymatic reactions required in cDNA synthesis. Along with the low concentrations of RNA present within lichen tissues, the process of creating a cDNA library is technically challenging.
Findings:
An evaluation of existing commercial and published protocols for RNA extraction from plant or fungal tissues has been performed and experimental conditions have been optimised to balance the need for the highest quality total ribonucleotides and the constraints of budget, time and human resources.
Conclusion:
We present a protocol that balances inexpensive RNA extraction methods with commercial RNA clean-up kits to yield sufficient RNA for cDNA library construction. Evaluation of the protocol and the construction of, and sampling from, a cDNA library is used to demonstrate the suitability of the RNA extraction method for expressed sequence tag production.
