Related Experiment Video
Updated: Jun 19, 2026
06:31
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
Published on: March 24, 2023
Bioinformatics construction of the human cell surfaceome
J P C da Cunha1, P A F Galante, J E de Souza
1São Paulo Branch, Ludwig Institute for Cancer Research, 01323-903 São Paulo, Brazil.
Summary
Researchers cataloged 3,702 human cell surface proteins (cell surfaceome) to identify potential diagnostic and therapeutic targets for cancer. This resource aids in discovering new cell surface cancer targets.
Area of Science:
- Genomics
- Proteomics
- Bioinformatics
Background:
- Cell surface proteins are crucial targets for medical interventions.
- A comprehensive catalog of these proteins is needed for cancer research.
Purpose of the Study:
- To create a catalog of the human cell surfaceome.
- To identify cell surface proteins with potential as diagnostic and therapeutic targets, particularly for tumors.
- To analyze the genetic diversity and expression patterns of the cell surfaceome.
Main Methods:
- Bioinformatics tools were used to generate the human cell surfaceome catalog.
- Gene expression data from various sources were integrated.
- Quantitative real-time PCR was employed for validation of selected surfaceome genes.
Main Results:
- A catalog of 3,702 human cell surface proteins was generated.
- Genes with restricted normal tissue expression and/or differential tumor expression were identified.
- Potential diagnostic and therapeutic targets for colorectal tumors and glioblastoma were discovered.
- Several cell surface cancer/testis antigens were identified.
Conclusions:
- The human cell surfaceome catalog provides a valuable reference for identifying cell surface targets.
- This resource facilitates the discovery of novel biomarkers and therapeutic strategies for various cancers.
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