Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Echinococcus multilocularis: characterization of a DNA probe.

M Vogel1, N Müller, B Gottstein

  • 1Institute of General Microbiology, University of Berne, Switzerland.

Acta Tropica
|December 1, 1990
PubMed
Summary

Researchers developed a DNA probe (pAL1) specific to Echinococcus multilocularis and Echinococcus granulosus. This probe allows for genetic discrimination between these cestode species and detection of genetic variation within E. multilocularis isolates.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis.

Open forum infectious diseases·2022
Same author

Elevated incidence of alveolar echinococcosis in immunocompromised patients.

Food and waterborne parasitology·2020
Same author

Apparent prevalence of and risk factors for infection with Ostertagia ostertagi, Fasciola hepatica and Dictyocaulus viviparus in Swiss dairy herds.

Veterinary parasitology·2018
Same author

Dogs as victims of their own worms: Serodiagnosis of canine alveolar echinococcosis.

Parasites & vectors·2017
Same author

Immunology of Alveolar and Cystic Echinococcosis (AE and CE).

Advances in parasitology·2017
Same author

Public Health Follow-up of Suspected Exposure to Echinococcus multilocularis in Southwestern Ontario.

Zoonoses and public health·2016

Area of Science:

  • * Molecular parasitology
  • * Helminth genetics
  • * Echinococcosis research

Background:

  • * Echinococcus multilocularis is a significant human and animal pathogen.
  • * Accurate species identification and genetic characterization are crucial for disease control.
  • * Existing diagnostic methods may lack specificity or resolution.

Purpose of the Study:

  • * To isolate and characterize a species-specific DNA fragment from E. multilocularis.
  • * To develop a molecular probe for differentiating Echinococcus species.
  • * To assess genetic variability within E. multilocularis populations.

Main Methods:

  • * Isolation of a 0.6 kb DNA fragment from E. multilocularis genomic DNA.
  • * Subcloning into a plasmid vector (pAL1) for use as a recombinant DNA probe.

Related Experiment Videos

  • * Biotinylation of the probe for Southern blot hybridization.
  • * Hybridization against genomic DNA from various cestode species, including E. multilocularis and E. granulosus.

    • Main Results:

    • * The pAL1 probe demonstrated strong hybridization to E. multilocularis DNA.
    • * Hybridization revealed specificity for E. multilocularis and E. granulosus.
    • * Distinct banding patterns allowed clear genomic-level discrimination between E. multilocularis and E. granulosus.
    • * The probe detected genetic variation among different E. multilocularis isolates.

    Conclusions:

    • * The pAL1 DNA probe is a valuable tool for specific identification of E. multilocularis and E. granulosus.
    • * This probe facilitates differentiation between closely related Echinococcus species at the genomic level.
    • * pAL1 aids in studying genetic diversity within E. multilocularis, relevant for epidemiological and control strategies.