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Related Experiment Video

Updated: Jun 19, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

p53 negatively regulates expression of FoxM1.

Bulbul Pandit, Marianna Halasi, Andrei L Gartel

    Cell Cycle (Georgetown, Tex.)
    |October 7, 2009
    PubMed
    Summary

    The tumor suppressor p53 normally downregulates the oncogenic transcription factor Forkhead box M1 (FoxM1). Inactivation of p53 in cancer cells leads to increased FoxM1, explaining its overexpression in tumors.

    Area of Science:

    • Molecular Biology
    • Oncology
    • Cancer Research

    Background:

    • Forkhead box M1 (FoxM1) is an oncogenic transcription factor frequently overexpressed in human cancers.
    • p53 is a critical tumor suppressor transcription factor, mutated in approximately 50% of human malignancies.
    • Understanding the regulatory relationship between p53 and FoxM1 is crucial for cancer biology.

    Discussion:

    • This study investigated the regulatory role of p53 on FoxM1 expression in human fibroblasts and cancer cell lines.
    • Experiments involved p53 knockdown and inactivation in BJ fibroblasts and wt-p53 cancer cell lines.
    • The impact of DNA-damage-induced p53 activation on FoxM1 levels was also assessed.

    Key Insights:

    • Partial deletion or inactivation of p53 results in the upregulation of FoxM1 expression.

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  • Knockdown of p53 in cancer cell lines with wild-type p53 leads to increased FoxM1 mRNA and protein.
  • p53 activation via DNA damage causes a downregulation of FoxM1 expression.

    • Outlook:

    • These findings establish a negative regulatory role for p53 on FoxM1 expression.
    • p53 inactivation in tumors may be a contributing factor to the observed overexpression of FoxM1.
    • Further research could explore therapeutic strategies targeting the p53-FoxM1 axis in cancer treatment.