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Updated: Jun 19, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Synthesis of multiantennary complex type N-glycans by use of modular building blocks
Carlo Unverzagt1, Gislinde Gundel, Steffen Eller
1Bioorganische Chemie, Universität Bayreuth, Gebäude NW1, 95440 Bayreuth, Germany. carlo.unverzagt@uni-bayreuth.de
Abstract:
A modular set of oligosaccharide building blocks was developed for the synthesis of multiantennary N-glycans of the complex type, which are commonly found on glycoproteins. The donor building blocks were laid out for the elongation of a core trisaccharide acceptor (beta-mannosyl chitobiose) conveniently protected with a single benzylidene moiety at the beta-mannoside. Through two consecutive regio- and stereoselective couplings the donors gave N-glycans with three to five antennae in high yields. Due to the consistent protection group pattern of the donors the deprotection of the final products can be performed by using a general reaction sequence.
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