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Updated: Jun 19, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
[Triterpenes from fruits of Paliurus ramosissimus]
Lei Yu1, Jing-Zhi Yang, Dong-Ming Zhang
1Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Abstract:
To study the chemical constituents of the fruits of Paliurus ramosissimus, various chromatographic techniques were used to separate and purify the chemical constituents. Three triterpenes have been isolated and purified by using various column chromatography. Their structures were elucidated by their physico-chemical properties and spectroscopic data. These compounds were determined as: 22S, 23R-epoxy-tirucalla-7-ene-3alpha,24, 25-triol (1), 21S, 23R-epoxy-21, 24S, 25-trihydroxy-apotirucalla-7-ene-3-one (2), 21R, 23R-epoxy-21-ethoxy-24S, 25-dihydroxy-apotirucalla-7-ene-3-one (3), separately. Compound 1 is a new compound, and the others were obtained from this genus for the first time.
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