Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

5-S-cysteinyldopac in human urine.

R Carstam1, C Brinck, B Fornstedt

  • 1Department of Dermatology, University of Lund, Sweden.

Acta Dermato-Venereologica
|January 1, 1990
PubMed
Summary

5-S-Cysteinyldopac, previously found only in brain tissue, is now identified in human urine. This discovery suggests non-specific oxidation of catechol derivatives in vivo, impacting pigment metabolism studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anal sac secretion in mustelids a comparison.

Journal of chemical ecology·2014
Same author

Marking urine and preputial gland secretion of male bank voles (Clethrionomys glareolus L.) chemical analyses and behavioral tests.

Journal of chemical ecology·2013
Same author

Dissection of the enzymatic and immunologic functions of macrophage migration inhibitory factor. Full immunologic activity of N-terminally truncated mutants.

European journal of biochemistry·2000
Same author

Inhibition of serum diamine oxidase discloses a constitutive putrescine release from cultured vascular smooth muscle cells.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·1999
Same author

Reducing compounds initiate the TRP2-catalyzed conversion of L-dopachrome to DHICA.

Melanoma research·1998
Same author

The protein catalysing the conversion of D-dopachrome to 5,6-dihydroxyindole is a phenylpyruvate tautomerase (EC 5.3.2.1)

Melanoma research·1998

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Human Physiology

Background:

  • 5-S-Cysteinyldopac is a known compound primarily identified in brain tissue.
  • Understanding the presence and origin of metabolites is crucial for interpreting biochemical pathways.
  • Catechol derivative metabolism plays a role in various physiological and pathological processes.

Purpose of the Study:

  • To isolate and quantify 5-S-Cysteinyldopac in human urine.
  • To investigate the formation pathway of 5-S-Cysteinyldopac in vivo.
  • To assess the implications of these findings for pigment metabolism research.

Main Methods:

  • Isolation and quantification of 5-S-Cysteinyldopac from human urine samples.
  • Incubation experiments involving 5-S-cysteinyldopamine and monoamine oxidase (MAO)-containing tissue.
  • Analysis to determine the direct formation of 5-S-Cysteinyldopac.

Main Results:

  • 5-S-Cysteinyldopac was successfully isolated and quantified in the urine of 12 individuals.
  • Average urinary excretion was measured at 20 +/- 9.1 micrograms/24 hours.
  • Incubation studies ruled out MAO-mediated formation, indicating direct nucleophilic addition of cysteine to dopac.

Conclusions:

  • 5-S-Cysteinyldopac is present in human urine, extending its known biological distribution.
  • The compound is formed via direct addition of cysteine to dopac, not through MAO activity.
  • These findings highlight non-specific oxidation of endogenous catechol derivatives in vivo, which must be considered in pigment metabolism studies using 5-S-cysteinyldopa.

Related Experiment Videos