Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of amino acid sequence and pH on nanofiber formation of self-assembling peptides EAK16-II and EAK16-IV.

Biomacromolecules·2003

Same author

The laparoscopic experience of surgical graduates in the United States.

Surgical endoscopy·2003

Same author

[Raman analysis of SiGe films grown by UHV/CVD].

Guang pu xue yu guang pu fen xi = Guang pu·2003

Same author

[Strengthening the epidemiological study on asthma].

Zhonghua nei ke za zhi·2003

Same author

[Isobutyramide activates transcription of human fetal gamma-globin gene selectively].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2003

Same author

Isobutyramide activates transcription of human fetal gamma- and murine embryonic epsilon(y)-globin genes.

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih·2003

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

Improved enzyme immobilization on an ionic-complementary peptide-modified electrode for biomolecular sensing.

Zhenyu Qian¹, Mushfique A Khan, Susan Mikkelsen

¹Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Langmuir : the ACS Journal of Surfaces and Colloids

|October 9, 2009

Summary

This study developed a new method for creating electrochemical biosensors using self-assembling peptides. The improved technique enhances enzyme immobilization, leading to significantly increased sensitivity and signal intensity for biosensing applications.

More Related Videos

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Related Experiment Videos

Last Updated: Jun 19, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Area of Science:

Electrochemistry
Biomaterials Science
Nanotechnology

Background:

Ionic-complementary peptides offer unique self-assembly properties for surface modification.
Previous methods for peptide immobilization on electrodes faced challenges with cross-linking and enzyme loading.

Purpose of the Study:

To optimize the immobilization of ionic-complementary peptides on highly ordered pyrolytic graphite (HOPG) electrodes for enhanced biosensor performance.
To develop a novel strategy to prevent peptide cross-linking during enzyme immobilization.

Main Methods:

Modification of HOPG electrode surface with ionic-complementary peptide EFK16-II.
Characterization of peptide nanofiber structure using atomic force microscopy (AFM).
Analysis of peptide cross-linking using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR).
Development of a premixing method for carbodiimide and glucose oxidase (GOx).

Main Results:

Self-assembling peptide EFK16-II formed a nanofiber structure on the HOPG electrode.
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) induced undesirable cross-linking of EFK16-II, reducing available carboxyl groups.
The premixing method successfully inhibited peptide cross-linking and improved GOx immobilization.
Biosensors exhibited a 6-fold increase in sensitivity (4.94 mA M(-1) cm(-2)) compared to previous designs.

Conclusions:

The developed premixing strategy significantly enhances enzyme immobilization on peptide-modified electrodes.
Ionic-complementary peptides show great promise for the development of highly sensitive electrochemical biosensors.
This approach offers a pathway to improved biosensor performance through optimized surface modification.