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Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Published on: January 2, 2013
Erythrocyte-endothelium adhesion can be induced by dextran
Yang Yang1, Huiling Eng, Björn Neu
1Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 639798.
Langmuir : the ACS Journal of Surfaces and Colloids
|October 9, 2009
Summary
Macromolecular depletion, mimicking plasma proteins, significantly increases red blood cell (RBC) adhesion to endothelial cells (EC). This suggests depletion interactions explain abnormal RBC-EC adhesion in diseases.
Area of Science:
- Biophysics
- Hematology
- Vascular Biology
Background:
- Plasma proteins are implicated in increased red blood cell (RBC) and endothelial cell (EC) adhesion in vascular diseases.
- The precise mechanisms by which plasma proteins mediate this adhesion are not fully understood.
Purpose of the Study:
- To investigate whether macromolecular depletion can induce RBC adhesion to EC.
- To elucidate the role of depletion interactions in RBC-EC adhesion.
Main Methods:
- Red blood cells (RBCs) were suspended in solutions with dextran to simulate nonadsorbing macromolecules.
- RBC-EC adhesion dynamics were quantified using a parallel plate flow chamber system.
Main Results:
- Dextran molecules with molecular mass >70 kDa markedly increased RBC adhesion to EC.
- Both the number of adhering cells and the strength of adhesion were significantly enhanced.
- This effect is attributed to depletion interactions caused by reduced polymer surface concentrations.
Conclusions:
- Macromolecular depletion, mimicking nonadsorbing plasma proteins, is a key factor in inducing RBC-EC adhesion.
- Depletion interactions provide a mechanistic explanation for abnormal RBC-EC adhesion observed in vivo.
- This finding offers insights into vascular complications in various diseases.
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