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Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Related Experiment Video

Updated: Jun 19, 2026

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Published on: March 3, 2017

Cell-type-specific proteome and interactome: using HIV-1 Tat as a test case.

Rachel Van Duyne¹, Kylene Kehn-Hall, Lawrence Carpio

¹The George Washington University, Department of Microbiology, Immunology and Tropical Medicine, 2300 I Street, NW, Washington, DC 20037, USA.

Expert Review of Proteomics

|October 9, 2009

Summary

Human immunodeficiency virus type 1 (HIV-1) complexity arises from cell-specific protein interactions and modifications. Understanding these interactions is key to controlling HIV-1 replication and latency.

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Last Updated: Jun 19, 2026

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Published on: March 3, 2017

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

Area of Science:

Virology
Molecular Biology
Immunology

Background:

Human immunodeficiency virus type 1 (HIV-1) controls host cells despite a limited proteome.
HIV-1 extensively interacts with host proteins, influencing cellular processes.
The complexity of HIV-1's host interactions requires further investigation beyond simple protein-protein interactions.

Purpose of the Study:

To explore the complexity of HIV-1 interactions with host cells.
To investigate the role of cell-type-specific interactions and post-translational modifications in HIV-1 control.
To examine the influence of HIV-1 Tat on viral transcription, considering these factors.

Main Methods:

Analysis of HIV-1 protein-protein interactions.
Investigation of cell-type-specific binding partners.
Study of post-translational modifications (acetylation, methylation) of viral proteins.
Assessment of subcellular localization effects on protein interactions.

Main Results:

HIV-1's control is attributed to numerous viral-host protein partnerships.
Cell-type-specific interactions and post-translational modifications significantly contribute to HIV-1 complexity.
HIV-1 Tat's function is modulated by cell type, post-translational modifications, and localization.

Conclusions:

HIV-1 complexity is not solely due to the number of protein interactions.
Cell-specific interactions and post-translational modifications are crucial for understanding HIV-1's host control.
Further research into these nuanced interactions is vital for therapeutic strategies against HIV-1.