Related Experiment Video
Updated: Jun 19, 2026
Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice
Published on: March 31, 2023
Methanol extract of Phellodendri cortex alleviates lipopolysaccharide-induced acute airway inflammation in mice
Yan-Fei Mao1, Yong-Qi Li, Lin Zong
1Departments of Surgical Intensive Care Unit, Xinhua Hospital,Shanghai Jiaotong University School of Medicine, Shanghai, P. R. China.
Background And Aim:
The effects of methanol extract of Phellodendri cortex on acute airway inflammation induced by intranasal administration of lipopolysaccharide (LPS, 300mug/kg) were investigated in female BALB/c mice.
Materials And Methods:
At 2 h after LPS exposure, mice were treated orally with methanol extract of Phellodendri cortex (100, 200 and 400 mg/kg). At the end of this study, bronchoalveolar lavage fluids (BALF) were collected and number of total cells, macrophages and neutrophils, protein concentration were analyzed. Tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein (MIP-2), IL-10 levels and nitric oxide (NO) production in BALF were also determined.
Results:
Methanol extract of Phellodendri cortex dose-dependently alleviated LPS-induced acute airway inflammation via decreasing the infiltration of inflammatory cells and the release of inflammatory mediators.
Conclusion:
The relief of airway inflammation provides a possible therapeutic application of Phellodendri cortex for the treatment of infectious pulmonary diseases.
