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Apototic cell-derived membrane vesicles induce CD83 expression on human mdDC

Eva-Marie Fehr1, Sonja Kierschke, Regina Max

  • 1Department of Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, 69120, Heidelberg, Germany.

Autoimmunity
|October 9, 2009
PubMed

Insights

Apoptotic cell-derived membrane vesicles (ACdMV) accumulate in autoimmune diseases due to defective phagocytosis. These vesicles interact with dendritic cells (DCs), potentially driving inflammation.

Area of Science:

  • Immunology
  • Cell Biology
  • Autoimmune Diseases

Background:

  • Apoptotic cell death involves shedding membrane vesicles, also known as membrane blebbing.
  • These vesicles contain autoantigens and are normally cleared by phagocytes.
  • Defective phagocytosis is implicated in autoimmune diseases like systemic lupus erythematosus.

Purpose of the Study:

  • To investigate the interaction between apoptotic cell-derived membrane vesicles (ACdMV) and myeloid dendritic cells (DCs).

Main Methods:

  • ACdMV were isolated from apoptotic lymphocytes.
  • Isolated ACdMV were morphologically characterized (average size 500 nm).
  • ACdMV were co-incubated with immature dendritic cells (iDCs).

Main Results:

  • ACdMV were characterized as membrane-coated vesicles.
  • Co-incubation with iDCs induced CD83 surface expression on dendritic cells.
  • Accumulation of ACdMV may contribute to inflammatory immune responses.

Conclusions:

  • Apoptotic cell-derived membrane vesicles interact with dendritic cells.
  • This interaction, particularly in the context of defective phagocytosis, may promote inflammation in autoimmune diseases.

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