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Updated: Jun 19, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
Published on: January 27, 2023
The 12/15-lipoxygenase pathway promotes osteoclast development and differentiation
Gerhard Krönke1, Stefan Uderhardt, Julia Katzenbeisser
1Department of Internal Medicine 3, Institute for Clinical Immunology, University of Erlangen-Nuremberg, 91054, Erlangen, Germany. gerhard.kroenke@uk-erlangen.de
Abstract:
Although 12/15-lipoxygenase (12/15-LO) has been implicated as negative regulator of systemic bone mass in mice and humans, the underlying mechanisms remain elusive. Here, we show that 12/15-LO is a positive regulator of osteoclast (OC) development. Enzymatic inhibition as well as genetic ablation of 12/15-LO significantly impaired osteoclastogenesis. Conversely, addition of the 12/15-LO-derived eicosanoids 12- and 15-HETE augmented differentiation of precursors into fully matured OCs. Together these data point towards a crucial role of 12/15-LO in the regulation of OC development. Therefore, 12/15-LO and its human homologue 15-LO may display novel targets for the treatment of diseases such as osteoporosis and rheumatoid arthritis.
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