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Updated: Jun 19, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
[Preparation and characterization of a novel functional anti-human CD83 monoclonal antibody]
Chao Gao1, Wei-Xue Zhong, Shu-Dan Zheng
1Institute of Medical Biotechnology, Soochow University, Suzhou 215007, China. ycgaochao@126.com
Aim:
To prepare and characterize a novel anti-human CD83 monoclonal mAb.
Methods:
Female BALB/c mice of 6-8 weeks old were immunized with CD83 transfectant (L929/CD83) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells.The hybridoma cells were screened with CD83 transfectant (L929/CD83 and 293/CD83) by FCM. The biological characteristics of antibody were investigated by rapid isotyping analysis, karyotype analysis, competitive inhibition test and Western blot.
Results:
One hybridoma cell line named 9D8 was obtained, which had the property of secreting anti-human CD83 monoclonal antibody steadily, This mAb specifically recognized CD83 molecule expressed on human mature DC, activated T cells, and tumor cell line Daudi, myeloma cell line 8226.This mAb can recognize a distinct epitope from commercial mAb (HB15e).
Conclusion:
One hybridoma cell line has been developed successfully, which may lay a foundation for further study on the function of this molecule.
