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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
12:47

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

Published on: May 14, 2012

T lineage differentiation from induced pluripotent stem cells.

Fengyang Lei1, Rizwanul Haque, Lauren Weiler

  • 1Department of Microbiology & Immunology and Penn State Hershey Cancer Institute, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Cellular Immunology
|October 9, 2009
PubMed
Summary

Induced pluripotent stem (iPS) cells can differentiate into T lymphocytes. This study shows iPS cell-derived T cells are functional and can repopulate T cell populations in vivo.

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Area of Science:

  • Stem cell biology
  • Immunology
  • Developmental biology

Background:

  • Induced pluripotent stem (iPS) cells offer potential for regenerative medicine.
  • The capacity of iPS cells to differentiate into specific immune lineages, like T lymphocytes, requires further elucidation.

Purpose of the Study:

  • To investigate the potential of iPS cells to differentiate into functional T lymphocytes.
  • To confirm the T cell differentiation capacity of iPS cells in vitro and in vivo.

Main Methods:

  • Co-culturing iPS cells with OP9 cells expressing Delta-like 1 (DL1).
  • In vitro stimulation assays for cytokine production (IL-2, IFN-gamma).
  • Adoptive transfer into Rag-deficient mice to assess T cell reconstitution.

Main Results:

  • iPS cells successfully differentiated into T lymphocytes when co-cultured on OP9-DL1 cells.
  • iPS cell-derived T lymphocytes produced key cytokines (IL-2, IFN-gamma) upon stimulation.
  • Adoptive transfer of these cells restored T cell populations in immunodeficient mice.

Conclusions:

  • iPS cells possess the inherent ability to undergo T cell differentiation.
  • iPS cells can generate functional T lymphocytes capable of immune reconstitution.
  • This supports the use of iPS cells for generating T cells for therapeutic applications.