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Dopamine D1 receptor stimulation increases striatal acetylcholine release in the rat
G Damsma1, C S Tham, G S Robertson
1Department of Psychiatry, University of British Columbia, Vancouver, Canada.
European Journal of Pharmacology
|September 21, 1990
Summary
Selective D1 receptor agonists increase acetylcholine release in the striatum. D1 and D2 receptors play opposing roles in regulating this release, impacting motor control and cognition.
Area of Science:
- Neuroscience
- Pharmacology
Background:
- Striatal acetylcholine (ACh) plays a crucial role in motor control and cognitive functions.
- Dopamine receptors, particularly D1 and D2 subtypes, are key regulators of neurotransmitter release in the striatum.
Purpose of the Study:
- To investigate the effect of selective D1 receptor agonists on in vivo acetylcholine release in the striatum.
- To elucidate the opposing roles of D1 and D2 receptors in regulating striatal ACh release.
Main Methods:
- In vivo microdialysis was employed to measure extracellular ACh levels in the striatum.
- Administration of selective D1 receptor agonists ((+)-SKF 38393, CY 204-283) and antagonists (SCH 23390).
- D2 receptor antagonist (raclopride) was used to assess receptor interactions.
Main Results:
- Selective D1 receptor agonists (+)-SKF 38393 and CY 204-283 significantly increased striatal ACh release.
- The D1 receptor antagonist SCH 23390 blocked the effect of the D1 agonist (+/-)-SKF 38393.
- Co-administration of a D2 receptor antagonist with a D1 agonist further enhanced ACh release.
Conclusions:
- Activation of D1 receptors stimulates acetylcholine release in the striatum.
- D1 and D2 dopamine receptors exert opposing modulatory effects on striatal ACh release.
- These findings provide insights into the dopaminergic regulation of cholinergic neurotransmission in the basal ganglia.