Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 19, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Dcp1-bodies in mouse oocytes.

Adam Swetloff1, Beatrice Conne, Joachim Huarte

  • 1Department of Genetic Medicine and Development, Faculty of Medicine, University of Geneva, 1211 Geneva 4, Switzerland.

Molecular Biology of the Cell
|October 9, 2009
PubMed
Summary

Processing bodies (P-bodies) are key for mRNA regulation. In mouse oocytes, novel P-body-like structures called Dcp1-bodies form and disappear during maturation, indicating a role in oocyte gene expression control.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.

bioRxiv : the preprint server for biology·2026
Same author

Cannabis consumption is associated with altered steroid metabolism in young men.

Communications medicine·2026
Same author

Chemogenetic activation of oxytocinergic neurons modulates acute neuroinflammation and improves brain development after pediatric traumatic brain injury.

Brain, behavior, and immunity·2026
Same author

Prenatal occupational exposure to endocrine-disrupting chemicals during pregnancy and adult male reproductive hormones.

Reproductive biomedicine online·2026
Same author

Chemogenetic activation of oxytocinergic neurons rescues neural correlates of encephalopathy of prematurity in mice.

Neurobiology of disease·2025
Same author

STARD9 and CDK5RAP2-Novel Candidate Genes for 46,XY Complete Gonadal Dysgenesis.

International journal of molecular sciences·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Processing bodies (P-bodies) are cytoplasmic granules regulating mRNA fate.
  • P-body components are found in germ cell granules, but their role in oocytes is unknown.
  • Oocytes rely on translational control for gene expression changes.

Purpose of the Study:

  • To characterize RNA granules in mouse oocytes.
  • To investigate the presence and nature of P-body-like structures in oocytes.
  • To understand the dynamic changes of these structures during oocyte maturation.

Main Methods:

  • Immunofluorescence microscopy to detect P-body markers (Dcp1a, RAP55, Rck/p54).
  • Observation of Dcp1-bodies in arrested primary oocytes and during in vitro maturation.

More Related Videos

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Related Experiment Videos

Last Updated: Jun 19, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

  • Analysis of protein partners and sensitivity to siRNA/miRNA depletion and translational inhibitors.
  • Monitoring of EGFP-hDcp1a posttranslational modification during disassembly.

    • Main Results:

    • P-body-like foci, termed Dcp1-bodies, were identified in mouse oocytes.
    • Two distinct types of Dcp1-bodies were observed in primary oocytes, differing in size, protein partners, and inhibitor sensitivity.
    • Dcp1-bodies disassembled during in vitro meiotic maturation, with disappearance by the metaphase II stage.
    • Disassembly correlated with posttranslational modification of EGFP-hDcp1a.

    Conclusions:

    • Mouse oocytes contain distinct P-body-like structures (Dcp1-bodies) involved in translational control.
    • These Dcp1-bodies undergo dynamic changes and disassembly during meiotic maturation.
    • The findings shed light on RNA granule function and regulation in mammalian oocytes.