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Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...

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A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
17:42

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Published on: April 13, 2011

Islet assessment for transplantation.

Klearchos K Papas1, Thomas M Suszynski, Clark K Colton

  • 1Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, 55455, USA. papas006@umn.edu

Current Opinion in Organ Transplantation
|October 9, 2009
PubMed
Summary

New assays measuring mitochondrial function in intact islets show promise for assessing islet cell transplantation quality. These tests are more reliable and correlate with transplant success in mice, aiding clinical evaluation.

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Area of Science:

  • Cell Biology
  • Transplantation Science
  • Biomedical Engineering

Background:

  • Clinical islet cell transplantation requires robust assays to ensure the viability and potency of islet preparations.
  • Existing assays often fail to accurately reflect islet quality due to limitations in methodology.
  • Significant research has focused on developing and validating new assays over the past decade.

Purpose of the Study:

  • To review recent advancements in islet viability and potency assays for clinical transplantation.
  • To identify and highlight the most promising assays for characterizing islet preparations.
  • To assess the correlation of novel assays with transplant outcomes.

Main Methods:

  • Evaluation of assays based on membrane integrity, noting their limitations.
  • Assessment of assays requiring islet disaggregation, identifying associated challenges.
  • Focus on assays measuring mitochondrial function (membrane potential, bioenergetic status, oxygen consumption rate) in intact islets.

Main Results:

  • Membrane integrity assays do not accurately predict true islet viability.
  • Disaggregation of islets for assays leads to cell damage and loss.
  • Mitochondrial function assays, particularly oxygen consumption rate in intact islets, are most promising.
  • Quantitative oxygen consumption rate assays have been validated and correlated with transplant outcomes in a mouse model.

Conclusions:

  • Novel, sensitive, and reliable islet viability and potency tests have been developed.
  • Mitochondrial function assays demonstrate the greatest promise for assessing islet quality.
  • These promising assays correlate with transplant outcomes in preclinical models and are under clinical evaluation.