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Related Concept Videos

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Drug Distribution: Plasma Protein Binding

Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...

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[Progresses in recombinant human serum albumin].

Riga Manchu1, Yingxia Zhang, Yun Zhang

  • 1The College of Life Science and Agriculture, Hainan University, Haikou 570228, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
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Recombinant human serum albumin (rHSA) offers a safe and effective alternative to plasma-derived human serum albumin (pdHSA). This bioengineered product mimics pdHSA

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Area of Science:

  • Biotechnology
  • Biochemistry
  • Clinical Pharmacy

Background:

  • Human serum albumin (HSA) is crucial for clinical applications like managing shock and hypoalbuminemia.
  • Plasma-derived HSA (pdHSA) carries risks of viral or prion contamination.
  • Biological engineering has enabled the production of recombinant human serum albumin (rHSA).

Purpose of the Study:

  • To evaluate the safety and efficacy of recombinant human serum albumin (rHSA).
  • To compare rHSA with plasma-derived human serum albumin (pdHSA).

Main Methods:

  • Production of rHSA using biological engineering.
  • Preclinical and clinical trials to assess rHSA.
  • Structural and functional analysis of rHSA compared to pdHSA.

Main Results:

  • rHSA demonstrates structural and functional similarity to pdHSA.
  • Preclinical and clinical studies confirm the safety of rHSA.
  • Efficacy of rHSA in treating specific diseases has been validated.

Conclusions:

  • rHSA is a viable and safe alternative to pdHSA.
  • rHSA production addresses contamination concerns associated with pdHSA.
  • rHSA holds significant potential in clinical pharmacy practice.