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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

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Related Experiment Video

Updated: Jun 19, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
10:14

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Ingested double-stranded RNAs can act as species-specific insecticides.

Steven Whyard1, Aditi D Singh, Sylvia Wong

  • 1Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. whyard@cc.umanitoba.ca

Insect Biochemistry and Molecular Biology
|October 10, 2009
PubMed
Summary

Researchers developed species-specific insecticides using RNA interference (RNAi). Oral delivery of double-stranded (ds) RNA effectively targeted and killed specific insect pests, offering a safer alternative to traditional pesticides.

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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

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Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes
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Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

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Related Experiment Videos

Last Updated: Jun 19, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
10:14

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
08:26

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Published on: May 1, 2018

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes
07:48

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

Published on: March 1, 2022

Area of Science:

  • Entomology
  • Molecular Biology
  • Pest Management

Background:

  • Conventional insecticides often harm non-target organisms, posing ecological risks.
  • RNA interference (RNAi) offers sequence-specific gene silencing, a potential tool for targeted pest control.

Purpose of the Study:

  • To develop orally-delivered double-stranded (ds) RNAs for species-specific pest elimination.
  • To demonstrate the efficacy of RNAi in diverse insect species for pest control applications.

Main Methods:

  • Designing species-specific dsRNAs targeting essential genes like vATPase and gamma-tubulin.
  • Administering dsRNA orally to target species including fruit flies, flour beetles, pea aphids, and tobacco hornworms.
  • Utilizing liposome encapsulation for enhanced dsRNA uptake in certain species like Drosophila.

Main Results:

  • Selective killing of target insect species (Drosophila melanogaster, Tribolium castaneum, Acyrthosiphon pisum, Manduca sexta) via ingested dsRNA.
  • Successful targeting of closely related species using dsRNAs against variable gene regions (3' UTRs).
  • Demonstrated RNAi induction through ingestion across multiple insect orders.

Conclusions:

  • Oral delivery of dsRNA is a viable method for species-specific insect control.
  • This approach shows promise for high-throughput RNAi screening and developing next-generation, eco-friendly insecticides.