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Updated: Jun 19, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
Published on: January 24, 2017
A microscale approach for predicting the performance of chromatography columns used to recover therapeutic polyclonal
Sunil Chhatre1, Daniel G Bracewell, Nigel J Titchener-Hooker
1The Advanced Centre for Biochemical Engineering, University College London, Gower Street, London WC1E 7JE, UK. sunil.chhatre@ucl.ac.uk
Abstract:
A microscale approach is described which screens conditions for recovering polyclonal antibodies from ovine sera by mixed-mode cation-exchange chromatography. The impact of pH and loading buffer salt concentration were assessed using robotically operated 20microL packed pipette tips. Low salt concentrations delivered capacities up to 41mg/mL, while only half this level was obtained at high salt concentrations. Two of the screened conditions were then tested in a 10mL packed bed and overall trends in capacity, yield and purity were found to be retained. Microscale pipette tips thus provided a useful basis for the rapid, approximate definition of a chromatography design space.
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