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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Rational and systematic protein purification process development: the next generation.

Beckley K Nfor¹, Peter D E M Verhaert, Luuk A M van der Wielen

¹Department of Biotechnology, Delft University of Technology, 2628 BC Delft, the Netherlands.

Trends in Biotechnology

|October 10, 2009

Summary

This summary is machine-generated.

Novel bioprocess development uses biothermodynamics and simulation tools to create databases for in silico purification. This enables faster response to product quality and market demands in biopharmaceutical manufacturing.

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Area of Science:

Biopharmaceutical Manufacturing
Process Development
Biothermodynamics

Background:

Current biopharmaceutical manufacturing relies on platform purification processes for speed and harmonization.
These platform processes face challenges in meeting increasing quality and capacity demands.

Purpose of the Study:

To introduce novel approaches for purification process development.
To enable faster and more informed process development for biopharmaceuticals.

Main Methods:

Incorporation of biothermodynamics principles.
Utilization of modern high-throughput experimentation (HTE).
Application of simulation tools for process modeling.

Main Results:

Development of production platform-specific databases.
Inclusion of thermodynamic protein descriptors for host cell proteins.
Creation of a foundation for in silico purification process development.

Conclusions:

The novel approach enhances process understanding.
It provides the potential for rapid adaptation to product quality and market needs.
Future work aims to further rationalize bioprocess development.