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Related Experiment Videos

Mesangial cell accessory functions: mediation by intercellular adhesion molecule-1.

D C Brennan1, A M Jevnikar, F Takei

  • 1Laboratory of Immunogenetics and Transplantation, Brigham and Women's Hospital Boston, Massachusetts.

Kidney International
|December 1, 1990
PubMed
Summary

Mesangial cells (MCs) can act as antigen-presenting cells (APCs) in glomerulonephritis. Cytokine stimulation enhances their ability to present antigens, contributing to kidney inflammation and damage.

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Area of Science:

  • Nephrology
  • Immunology
  • Cell Biology

Background:

  • Mesangial cell (MC) proliferation is a key feature of immune-mediated glomerulonephritis.
  • MC position and inflammatory molecule production suggest a role in initiating glomerular damage.
  • Increased cytokines, MHC class II, and ICAM-1 are observed in lupus nephritis.

Purpose of the Study:

  • To investigate if mesangial cells can function as antigen-presenting cells (APCs) or accessory cells after cytokine stimulation.
  • To determine the role of cytokines like IFN-gamma, TNF, and IL-1 in modulating MCs' APC capabilities.

Main Methods:

  • Established a permanent mesangial cell (MC) line using SV40 DNA transformation.
  • Analyzed surface expression of ICAM-1 and MHC class II molecules on MCs.

Related Experiment Videos

  • Stimulated MCs with cytokines (IFN-gamma, TNF, IL-1) and assessed antigen presentation to T cell hybridomas.
  • Utilized monoclonal antibodies (mAbs) against ICAM-1 and MHC class II to block cellular interactions.

    • Main Results:

    • Intercellular Adhesion Molecule 1 (ICAM-1) expression was upregulated by IFN-gamma, TNF, and IL-1.
    • Major Histocompatibility Complex (MHC) class II expression required prolonged IFN-gamma stimulation.
    • Cytokine-stimulated MCs adhered to and presented antigens to T cells.
    • Anti-ICAM-1 mAb reduced MC adhesion and antigen presentation; anti-MHC class II mAb blocked antigen presentation but not adhesion.

    Conclusions:

    • Mesangial cells can be induced to function as antigen-presenting cells (APCs) by specific cytokines.
    • Both ICAM-1 and MHC class II expression are crucial for MC-mediated antigen presentation.
    • These findings highlight MCs as potential key players in the immune response within the glomerulus.