Related Experiment Video
Updated: Jun 19, 2026

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
Published on: November 1, 2012
Acute passive stretching in a previously fatigued muscle: Electrical and mechanical response during tetanic
Fabio Esposito1, Emiliano Ce, Susanna Rampichini
1Department of Sport, Nutrition and Health Sciences, University of Milan, Italy. fabio.esposito@unimi.it
Abstract:
The aim of this study was to assess the effects of acute passive stretching on the electrical and mechanical response of a previously fatigued muscle. Eleven participants underwent maximal tetanic stimulations (50 Hz) of the medial gastrocnemius, before and after a fatiguing protocol and after a bout of passive stretching of the fatigued muscle. During contraction, surface electromyography (EMG), mechanomyography (MMG), and force were recorded. The following parameters were calculated: (1) the EMG root mean square (RMS), mean frequency, and fibre conduction velocity; (2) MMG peak-to-peak and RMS; (3) the peak force, contraction time, half-relaxation time, peak rate of force development (dF/dt) and its acceleration (d(2)F/dt(2)). Fatigue reduced peak force by 18% (P < 0.05) and affected the other force, EMG, and MMG parameters. After stretching: (1) all EMG parameters recovered to pre-fatigue values; (2) MMG peak-to-peak remained depressed, while RMS recovered to pre-fatigue values; (3) the peak force, peak rate of force development and its acceleration were further reduced by 22, 18, and 51%, respectively, and half-relaxation time by 40% (P < 0.05). In conclusion, acute passive stretching, when applied to a previously fatigued muscle, further depresses the maximum force-generating capacity. Although stretching does not alter the electrical parameters of the fatigued muscle, it does affect the mechanical behaviour of the muscle-tendon unit.
Related Concept Videos
Muscle Stimulation Frequency
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Relaxation of Skeletal Muscles
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
Motor Unit Stimulation
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
Somatic Spinal Reflexes
One of the most well-known somatic spinal reflexes is the stretch reflex, which is activated by the sudden stretching of a muscle. This reflex involves the activation of specialized sensory receptors called muscle spindles, which are located in the muscle tissue and detect changes in the length and speed of muscle contractions. When a muscle is suddenly...
Tetanus
Excitation-Contraction Coupling in Skeletal Muscles
When an action potential...

