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Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

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Updated: Jun 19, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Gene expression in stem cells.

Yu Liang1, Iain Russell, Criss Walworth

  • 1Molecular Biology Systems Division, Life Technologies, Foster City, CA 94404, USA.

Critical Reviews in Eukaryotic Gene Expression
|October 13, 2009
PubMed
Summary
This summary is machine-generated.

Gene expression analysis advances stem cell research for degenerative diseases and cancer. Understanding stemness is crucial for identifying cancer stem cell markers and improving clinical applications.

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Related Experiment Videos

Last Updated: Jun 19, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Area of Science:

  • Stem cell biology
  • Cancer biology
  • Genomics

Background:

  • Embryonic and adult stem cells offer potential for treating degenerative diseases.
  • Understanding stemness (stem cell biology) is key for clinical applications.
  • Gene expression analysis has been vital in characterizing stem cell function.

Purpose of the Study:

  • To review advances in gene expression analysis for stem cell research.
  • To explore the role of gene expression in cancer stem cells.
  • To identify challenges in finding cancer stem cell markers.

Main Methods:

  • Genome-wide gene expression analysis.
  • Targeted gene expression analysis of specific gene subsets.
  • Review of recent literature on stem cell gene expression.

Main Results:

  • Gene expression profiling has identified molecular signatures of stem cell function.
  • Gene expression studies are unraveling the role of cancer stem cells in tumor initiation and progression.
  • Advances in gene expression analysis are improving the understanding of stemness.

Conclusions:

  • Gene expression analysis is a powerful tool in stem cell research.
  • Further research is needed to elucidate the role of cancer stem cells.
  • Identifying reliable cancer stem cell markers remains a critical challenge.