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Related Experiment Video

Updated: Jun 19, 2026

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

Islet protein profiling.

P Bergsten1

  • 1Department of Medical Cell Biology, Uppsala University, Sweden. Peter.Bergsten@mcb.uu.se

Diabetes, Obesity & Metabolism
|October 13, 2009
PubMed
Summary
This summary is machine-generated.

Islet protein profiling reveals complex changes in diabetes models, offering insights into islet cell dysfunction. This technique helps understand disease mechanisms and potential interventions when human samples are unavailable.

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Area of Science:

  • Endocrinology
  • Proteomics
  • Metabolic Diseases

Background:

  • Islet dysfunction is central to type 1 and type 2 diabetes mellitus.
  • Understanding islet cell protein expression is crucial for elucidating disease pathogenesis.
  • Limited direct protein profiling data exists for human diabetic islets.

Purpose of the Study:

  • To define islet protein profiling and its methodologies.
  • To review applications of protein profiling in diabetes research.
  • To highlight the utility of animal models for studying islet dysfunction.

Main Methods:

  • Protein profiling involves separation (gel-based or chromatography), differential expression determination, and protein identification via mass spectrometry.
  • Analysis of large datasets interprets protein changes into cellular mechanisms.

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Isolation of Human Islets from Partially Pancreatectomized Patients
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Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

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Last Updated: Jun 19, 2026

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

Isolation of Human Islets from Partially Pancreatectomized Patients
11:10

Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

  • Comparison of protein and transcript data reveals discordance, emphasizing the value of proteomic approaches.

    • Main Results:

    • Islet protein profiling generates comprehensive expression datasets from various cell types and models.
    • Studies have addressed islet dysfunction induced by hyperglycemia, lipotoxicity, and inflammation using this approach.
    • Animal models (BB-DP rats, NOD, ob/ob, MKR mice) have been instrumental in dissecting disease mechanisms.

    Conclusions:

    • Islet protein profiling is a powerful tool for understanding islet cellular events in diabetes.
    • The technique has elucidated mechanisms of islet dysfunction in diabetes models.
    • Findings provide avenues for therapeutic interventions in diabetes mellitus.