The application of oligonucleotide probes to HLA class II typing of the DRB sub-region
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Updated: Sep 12, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
R W Vaughan1, J S Lanchbury, S G Marsh
1Dept. Molecular Immunogenetics, Guy's Hospital, London, U.K.
Abstract:
A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.
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