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Related Experiment Video

Updated: Jun 19, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

A simple quantitative bedside test to determine methemoglobin.

Fathima Shihana¹, Dhammika Menike Dissanayake, Nicholas Allan Buckley

¹South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. shihana@sactrc.org

Annals of Emergency Medicine

|October 13, 2009

Summary

This summary is machine-generated.

A new low-cost bedside color chart accurately estimates methemoglobin levels in pesticide poisoning cases. This method is valuable for resource-limited settings lacking laboratory facilities.

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Area of Science:

Clinical Chemistry
Toxicology
Point-of-Care Diagnostics

Background:

Methemoglobinemia from pesticide poisoning has high mortality (12%) in Sri Lanka.
Limited laboratory facilities complicate treatment in resource-limited settings.

Purpose of the Study:

Develop and validate a low-cost, bedside test for quantitative methemoglobin estimation.
Improve clinical management of methemoglobinemia in resource-limited areas.

Main Methods:

Created a color chart using scanned methemoglobin blood samples (10-100%).
Measured red, green, and blue values using a flatbed scanner and ImageJ software.
Assessed interobserver agreement and compared with spectrometric measurements.

Main Results:

Red color value showed a strong linear correlation with methemoglobin percentage (R²=0.9938).
High interobserver agreement (94% and 88%) was achieved using the color chart.
Clinical use demonstrated good agreement with standard spectrometric methods.

Conclusions:

A color chart provides a clinically useful, quantitative estimate of methemoglobinemia.
This bedside test is suitable for resource-limited settings.