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Updated: Jun 19, 2026

Isolation of Mouse Megakaryocyte Progenitors
Published on: May 20, 2021
Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
Atsuko Masumi1, Isao Hamaguchi, Madoka Kuramitsu
1Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan. amasumi@nih.go.jp
Abstract:
Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-gamma treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.
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