The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors
View abstract on PubMed
Summary
This summary is machine-generated.Mapping nuclear receptors in the intestine reveals that their normal expression patterns predict changes in tumors. This intestinal nuclear hormone receptor map aids in identifying early diagnostic markers for colon cancer.
Area Of Science
- Gastroenterology
- Molecular Biology
- Oncology
Background
- The WNT-adenomatous polyposis coli (APC) pathway regulates intestinal epithelial cell fate.
- Nuclear receptors act as sensors for hormones and nutrients, influencing colon cancer progression.
- Understanding nuclear receptor expression is crucial for intestinal health and disease.
Purpose Of The Study
- To map messenger RNA (mRNA) abundance and epithelial localization of all nuclear receptors in mouse and human intestines.
- To compare nuclear receptor expression in normal versus neoplastic intestinal tissues.
- To establish a comprehensive overview of the intestinal nuclear hormone receptor transcriptome.
Main Methods
- High-resolution in situ hybridization and quantitative polymerase chain reaction (qPCR).
- Analysis of normal and tumor tissues from mouse models (Apc(Min/+)) and familial adenomatous polyposis patients.
- Utilized cellular models of human colon cancer.
Main Results
- Defined expression patterns for each nuclear receptor based on transcript localization in the ileum and colon.
- Identified differential mRNA levels between normal intestinal epithelium and neoplastic tissue.
- Developed classification trees for the nuclear hormone receptor intestinal transcriptome, showing mouse-human correspondence.
Conclusions
- Established the intestinal nuclear hormone receptor map.
- Demonstrated that normal receptor localization predicts tumor expression modulation.
- Results can guide selection of nuclear receptors for early diagnostic markers or therapeutic targets in intestinal polyposis and cancer.